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Brief Communication

Nature Methods 5, 227–229 (1 March 2008) | doi:10.1038/nmeth.1183

Tractable Cre-lox system for stochastic alteration of genes in mice

Ashleigh J Miller , Sandra D Dudley , Jen-Lan Tsao , Darryl Shibata & R Michael Liskay

We developed a cell division–activated Cre-lox system for stochastic recombination of loxP-flanked loci in mice. Cre activation by frameshift reversion is modulated by DNA mismatch-repair status and occurs in individual cells surrounded by normal tissue, mimicking spontaneous cancer-causing mutations.