Access

Brief Communication

Nature Methods 5, 231–233 (1 March 2008) | doi:10.1038/nmeth.1182

Generating somatic mosaicism with a Cre recombinase|[ndash]|microsatellite sequence transgene

Aytekin Akyol , Takao Hinoi , Ying Feng , Guido T Bommer , Thomas M Glaser & Eric R Fearon

Strategies for altering constitutional or somatic genotype in mice are well established, but approaches to generate mosaic genotypes in mouse tissues are limited. We showed that a functionally inactive Cre recombinase transgene with a long mononucleotide tract altering the reading frame was stochastically activated in the mouse intestinal tract.