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Article
Nature Methods - 5, 253 - 260 (2008)
Published online: 24 February 2008; | doi:10.1038/nmeth.1186

Homogeneous reporter system enables quantitative functional assessment of multiple transcription factors

Sergei Romanov1, Alexander Medvedev1, Maria Gambarian1, Natalia Poltoratskaya1, Matt Moeser1, Liubov Medvedeva1, Mikhail Gambarian2, Luda Diatchenko3 & Sergei Makarov1

1  Attagene Inc., P.O. Box 12054, Research Triangle Park, North Carolina 27709, USA.

2  MishaGam Software, 525 Riverside Ln., Cayce, South Carolina 29033, USA.

3  Center for Neurosensory Disorders, 2120 Old Dental Building, CB 7450, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.

Correspondence should be addressed to Sergei Makarov smak@attagene.com

We developed a high-content reporter system that allows quantitative assessment of activities of multiple transcription factors (TFs) in a eukaryotic cell. The system comprises a library of reporter constructs that are evaluated according to their transcription rates. All reporters produce essentially identical messages that are subjected to 'processing', which generates a spectrum of distinguishable fragments that are analyzed quantitatively. The homogeneity of the reporter library afforded inherently uniform detection conditions for all reporters and provided repeatability, accuracy and robustness of assessment. We showed that this technology can be used to identify pathways transmitting cell responses to inducers, and that the profile of TF activities generated using this system represents a stable and sustained cell signature that clearly distinguishes different cell types and pathological conditions. This technology provides a framework for functional characterization of signal transduction networks through profiling activities of multiple TFs.

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Nature Methods
ISSN: 1548-7091
EISSN: 1548-7105
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