Nature Methods
- 5, 227 - 229 (2008)
Published online: 10 February 2008; | doi:10.1038/nmeth.1183
Tractable Cre-lox system for stochastic alteration of genes in miceAshleigh J Miller1, Sandra D Dudley1, Jen-Lan Tsao2, Darryl Shibata2 & R Michael Liskay11
Molecular and Medical Genetics, L103, Oregon Health & Science University, 3181 Sam Jackson Park Road, Portland, Oregon 97239, USA. 2
Department of Pathology, University of Southern California Keck School of Medicine, 1200 N. State Street, Unit I, Room 2428, Los Angeles, California 90033, USA.
Correspondence should be addressed to R Michael Liskay liskaym@ohsu.edu We developed a cell division–activated Cre-lox system for stochastic recombination of loxP-flanked loci in mice. Cre activation by frameshift reversion is modulated by DNA mismatch-repair status and occurs in individual cells surrounded by normal tissue, mimicking spontaneous cancer-causing mutations. This system should be particularly useful for delineating pathways of neoplasia, and determining the developmental and aging consequences of specific gene alterations.
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