Nature Methods
- 5, 231 - 233 (2008)
Published online: 10 February 2008; | doi:10.1038/nmeth.1182
Generating somatic mosaicism with a Cre recombinase–microsatellite sequence transgeneAytekin Akyol1, 5, Takao Hinoi1, 5, Ying Feng1, Guido T Bommer1, Thomas M Glaser1, 2 & Eric R Fearon1, 2, 3, 41
Department of Internal Medicine, University of Michigan Medical School, 109 Zina Pitcher, Ann Arbor, Michigan 48109, USA. 2
Department of Human Genetics, University of Michigan Medical School, 109 Zina Pitcher, Ann Arbor, Michigan 48109, USA. 3
Department of Pathology, University of Michigan Medical School, 109 Zina Pitcher, Ann Arbor, Michigan 48109, USA. 4
The Cancer Center, University of Michigan Medical School, 109 Zina Pitcher, Ann Arbor, Michigan 48109, USA. 5
Present addresses: Hacettepe University School of Medicine Department of Pathology, 06100 Shihhiye, Ankara, Turkey (A.A.) and Department of Surgery, Hiroshima University, 1-2-3 Kasumi, Minami-ku Hiroshima 734-8551, Japan (T.H.).
Correspondence should be addressed to Eric R Fearon fearon@umich.edu Strategies for altering constitutional or somatic genotype in mice are well established, but approaches to generate mosaic genotypes in mouse tissues are limited. We showed that a functionally inactive Cre recombinase transgene with a long mononucleotide tract altering the reading frame was stochastically activated in the mouse intestinal tract. We demonstrated the utility of this approach by inducing colonic polyposis after Cre-mediated bi-allelic inactivation of the Apc gene.
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