Nature Methods
- 5, 175 - 177 (2008)
Published online: 20 January 2008; | doi:10.1038/nmeth.1178
Printing protein arrays from DNA arraysMingyue He1, 4, Oda Stoevesandt1, 3, 4, Elizabeth A Palmer1, 3, Farid Khan1, 3, Olle Ericsson2 & Michael J Taussig1, 31
Technology Research Group, The Babraham Institute, Cambridge CB22 3AT, UK. 2
Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, SE-75185 Uppsala, Sweden. 3
Present addresses: Protein Technology Group, Babraham Bioscience Technologies, Babraham Research Campus, Cambridge CB22 3AT, UK (O.S. and M.J.T.), MRC Virology Unit, Glasgow G11 5JR, Scotland, UK (E.A.P), and University of Manchester, Manchester Interdisciplinary Biocentre, Manchester, M1 7DN, UK (F.K.). 4
These authors contributed equally to this work.
Correspondence should be addressed to Michael J Taussig mike.taussig@bbsrc.ac.uk We describe a method, DNA array to protein array (DAPA), which allows the 'printing' of replicate protein arrays directly from a DNA array template using cell-free protein synthesis. At least 20 copies of a protein array can be obtained from a single DNA array. DAPA eliminates the need for separate protein expression, purification and spotting, and also overcomes the problem of long-term functional storage of surface-bound proteins.
MORE ARTICLES LIKE THIS These links to content published by NPG are automatically generated.
|
