Nature Methods
- 5, 171 - 173 (2008)
Published online: 20 January 2008; | doi:10.1038/nmeth.1177
A secreted luciferase for ex vivo monitoring of in vivo processesThomas Wurdinger1, 2, 3, Christian Badr1, Lisa Pike1, Ruben de Kleine2, Ralph Weissleder2, 4, Xandra O Breakefield1, 2, 3 & Bakhos A Tannous1, 2, 3, 51
Molecular Neurogenetics Unit, Department of Neurology, Harvard Medical School, 149 13th St., Charlestown, Massachusetts 02129, USA. 2
Center for Molecular Imaging Research, Department of Radiology, Harvard Medical School, 149 13th St., Charlestown, Massachusetts 02129, USA. 3
Program in Neuroscience, Harvard Medical School, 149 13th St., Charlestown, Massachusetts 02129, USA. 4
Center for Systems Biology, Massachusetts General Hospital, 185 Charles River Plaza, Boston, Massachusetts 02114, USA. 5
Department of Pathology, Massachusetts General Hospital, 55 Fruit St., Boston, Massachusetts 02114, USA.
Correspondence should be addressed to Bakhos A Tannous btannous@hms.harvard.edu Luciferases are widely used to monitor biological processes. Here we describe the naturally secreted Gaussia princeps luciferase (Gluc) as a highly sensitive reporter for quantitative assessment of cells in vivo by measuring its concentration in blood. The Gluc blood assay complements in vivo bioluminescence imaging, which has the ability to localize the signal and provides a multifaceted assessment of cell viability, proliferation and location in experimental disease and therapy models.
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