Nature Methods
- 5, 167 - 169 (2008)
Published online: 13 January 2008; | doi:10.1038/nmeth.1173
Plasmid-chromosome shuffling for non-deletion alleles in yeastZhiwei Huang1, 4, Richard S Sucgang1, 4, Yu-yi Lin3, Xiaomin Shi1, Jef D Boeke3 & Xuewen Pan1, 21
Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA. 2
Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA. 3
High Throughput Biology Center, The Johns Hopkins University School of Medicine, 733 N. Broadway, Baltimore, Maryland 21205, USA. 4
These authors contributed equally to this work.
Correspondence should be addressed to Jef D Boeke jboeke@jhmi.edu or Xuewen Pan xuewenp@bcm.edu Here we describe a facile plasmid-chromosome shuffling technique for generating and analyzing non-deletion alleles in the yeast Saccharomyces cerevisiae. This technique takes advantage of an existing set of genome-wide haploid-convertible heterozygous diploid yeast knockout mutants. This simple method will facilitate characterization of essential gene functions and genome-wide investigation of protein structure-function relationships.
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