Nature Methods
- 5, 179 - 181 (2008)
Published online: 13 January 2008; | doi:10.1038/nmeth.1172
Pyrobayes: an improved base caller for SNP discovery in pyrosequencesAaron R Quinlan, Donald A Stewart, Michael P Strömberg & Gábor T Marth
Department of Biology, Boston College, 140 Commonwealth Avenue, Chestnut Hill, Massachusetts 02467, USA.
Correspondence should be addressed to Gábor T Marth marth@bc.edu Previously reported applications of the 454 Life Sciences pyrosequencing technology have relied on deep sequence coverage for accurate polymorphism discovery because of frequent insertion and deletion sequence errors. Here we report a new base calling program, Pyrobayes, for pyrosequencing reads. Pyrobayes permits accurate single-nucleotide polymorphism (SNP) calling in resequencing applications, even in shallow read coverage, primarily because it produces more confident base calls than the native base calling program.
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