Perspective abstract

Nature Methods 5, 1005 - 1010 (2008)
Published online: 25 November 2008 | doi:10.1038/nmeth.1270

A large genome center's improvements to the Illumina sequencing system

Michael A Quail1, Iwanka Kozarewa1, Frances Smith1, Aylwyn Scally1, Philip J Stephens1, Richard Durbin1, Harold Swerdlow1 & Daniel J Turner1

The Wellcome Trust Sanger Institute is one of the world's largest genome centers, and a substantial amount of our sequencing is performed with 'next-generation' massively parallel sequencing technologies: in June 2008 the quantity of purity-filtered sequence data generated by our Genome Analyzer (Illumina) platforms reached 1 terabase, and our average weekly Illumina production output is currently 64 gigabases. Here we describe a set of improvements we have made to the standard Illumina protocols to make the library preparation more reliable in a high-throughput environment, to reduce bias, tighten insert size distribution and reliably obtain high yields of data.

  1. Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, CB10 1SA, UK.

Correspondence to: Daniel J Turner1 e-mail: