Perspective abstract
Nature Methods 5, 927 - 933 (2008)
Published online: 30 October 2008 | doi:10.1038/nmeth.1265
Native mass spectrometry: a bridge between interactomics and structural biology
Albert J R Heck1
Abstract
Native mass spectrometry is an emerging technology that allows the topological investigation of intact protein complexes with high sensitivity and a theoretically unrestricted mass range. This unique tool provides complementary information to established technologies in structural biology, and also provides a link to high-throughput interactomics studies, which do not generate information on exact protein complex-composition, structure or dynamics. Here I review the current state of native mass spectrometry technology and discuss several important biological applications. I also describe current experimental challenges in native mass spectrometry, encouraging readers to contribute to solutions.
- Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands.
Correspondence to: Albert J R Heck1 e-mail: a.j.r.heck@uu.nl
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