Brief Communication abstract
Nature Methods 5, 951 - 953 (2008)
Published online: 19 October 2008 | doi:10.1038/nmeth.1261
Linking SNPs to CAG repeat length in Huntington's disease patients
Wanzhao Liu1, Lori A Kennington1, H Diana Rosas2, Steven Hersch2, Jang-Ho Cha2, Phillip D Zamore3 & Neil Aronin1
Allele-specific silencing using small interfering RNAs targeting heterozygous single-nucleotide polymorphisms (SNPs) is a promising therapy for human trinucleotide repeat diseases such as Huntington's disease. Linking SNP identities to the two HTT alleles, normal and disease-causing, is a prerequisite for allele-specific RNA interference. Here we describe a method, SNP linkage by circularization (SLiC), to identify linkage between CAG repeat length and nucleotide identity of heterozygous SNPs using Huntington's disease patient peripheral blood samples.
- Department of Medicine, University of Massachusetts Medical School, 364 Plantation Street, Worcester, Massachusetts 01605, USA.
- MassGeneral Institute for Neurodegenerative Disease, Massachusetts General Hospital, 16th Street, Charlestown, Massachusetts 02129, USA.
- Department of Biochemistry and Molecular Pharmacology, and Howard Hughes Medical Institute, University of Massachusetts Medical School, 364 Plantation Street, Worcester, Massachusetts 01605, USA.
Correspondence to: Phillip D Zamore3 e-mail: phillip.zamore@umassmed.edu
Correspondence to: Neil Aronin1 e-mail: neil.aronin@umassmed.edu
MORE ARTICLES LIKE THIS
These links to content published by NPG are automatically generated.
NEWS AND VIEWS
Expanded CAG repeats in the crosshairsNature Biotechnology News and Views (01 May 2009)
Huntingtin's critical cleavageNature Neuroscience News and Views (01 Sep 2006)
RESEARCH
Allele-specific silencing of mutant huntingtin and ataxin-3 genes by targeting expanded CAG repeats in mRNAsNature Biotechnology Research (01 May 2009)
AAV Vector?mediated RNAi of Mutant Huntingtin Expression Is Neuroprotective in a Novel Genetic Rat Model of Huntington's DiseaseMolecular Therapy Original Article
See all 7 matches for Research
