Brief Communication abstract
Nature Methods 5, 947 - 949 (2008)
Published online: 15 September 2008 | doi:10.1038/nmeth.1258
Super-resolution imaging in live Caulobacter crescentus cells using photoswitchable EYFP
Julie S Biteen1, Michael A Thompson1, Nicole K Tselentis1, Grant R Bowman2, Lucy Shapiro2 & W E Moerner1
The commonly used, monomeric EYFP enabled imaging of intracellular protein structures beyond the optical resolution limit ('super-resolution' imaging) in living cells. By combining photoinduced activation of single EYFP fusions and time-lapse imaging, we obtained sub–40 nm resolution images of the filamentous superstructure of the bacterial actin protein MreB in live Caulobacter crescentus cells. These studies demonstrated that EYFP is a useful emitter for in vivo super-resolution imaging.
- Department of Chemistry, Stanford University, 375 North-South Mall, Stanford, California 94305, USA.
- Department of Developmental Biology, Stanford University, B300 Beckman Center, Stanford, California 94305, USA.
Correspondence to: W E Moerner1 e-mail: wmoerner@stanford.edu
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