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Nature Methods 5, 858–859 (1 October 2008) | doi:10.1038/nmeth1008-858b

Phosphorylation and the cell cycle

Allison Doerr

The role of phosphorylation in regulating protein function and cellular signaling is perhaps one of the most important questions in biology. Classical methods like mobility-shift assays allow researchers to identify whether a protein was phosphorylated under certain conditions, but it is extremely difficult to look at large-scale phosphorylation changes across the cell.