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Brief Communication
Nature Methods 5, 869–872 (1 October 2008) | doi:10.1038/nmeth.1250
Automated screening for mutants affecting dopaminergic-neuron specification in C. elegans
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Abstract
We describe an automated method to isolate mutant Caenorhabditis elegans that do not appropriately execute cellular differentiation programs. We used a fluorescence-activated sorting mechanism implemented in the COPAS Biosort machine to isolate mutants with subtle alterations in the cellular specificity of GFP expression.
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