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| Special Feature | Top |
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Nature Methods' Method of the Year 2007 goes to next-generation sequencing. This series of articles showcase how these novel sequencing methods came into their own in 2007 and the incredible impact they promise to have in a variety of research applications. The Methods to Watch feature provide a glimpse and a wish list for future Methods of the Year.
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| Editorial | Top |
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Special Feature: Method of the Year 2007
Method of the Year p1
doi:10.1038/nmeth1153
Abstract | Full text | PDF
(52K)
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| Correspondence | Top |
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Vector-free DNA constructs improve transgene expression in C. elegans p3
John F Etchberger & Oliver Hobert
doi:10.1038/nmeth0108-3
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(114K)
| Supplementary Information |
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| Research Highlights | Top |
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Inner glow p5
Katherine Stevens
doi:10.1038/nmeth0108-5
Abstract | Full text | PDF
(178K)
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Smaller can be better pp6 - 7
Allison Doerr
doi:10.1038/nmeth0108-6a
Abstract | Full text | PDF
(138K)
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There is a virus going around pp6 - 7
Katherine Stevens
doi:10.1038/nmeth0108-6b
Abstract | Full text | PDF
(138K)
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News in brief p7
doi:10.1038/nmeth0108-7
Full text | PDF
(80K)
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The living brain, unlabeled p8
Natalie de Souza
doi:10.1038/nmeth0108-8
Abstract | Full text | PDF
(132K)
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| News Feature | Top |
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Special Feature: Method of the Year 2007
The year of sequencing pp11 - 14
Kelly Rae Chi
doi:10.1038/nmeth1154
Abstract | Full text | PDF
(665K)
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| Primer | Top |
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Special Feature: Method of the Year 2007
Primer: Sequencing—the next generation p15
Nicole Rusk & Veronique Kiermer
Published online: 19 December 2007 | doi:10.1038/nmeth1155
Abstract | Full text | PDF
(349K)
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| Commentaries | Top |
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Special Feature: Method of the Year 2007
Next-generation sequencing transforms today's biology pp16 - 18
Stephan C Schuster
Published online: 19 December 2007 | doi:10.1038/nmeth1156
Abstract | Full text | PDF
(342K)
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Special Feature: Method of the Year 2007
Sequence census methods for functional genomics pp19 - 21
Barbara Wold & Richard M Myers
Published online: 19 December 2007 | doi:10.1038/nmeth1157
Abstract | Full text | PDF
(416K)
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| Methods to Watch | Top |
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Special Feature: Method of the Year 2007
Seeing fluorescence at super-resolution p22
Daniel Evanko
doi:10.1038/nmeth1158
Abstract | Full text | PDF
(606K)
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Special Feature: Method of the Year 2007
Experimental micro-matchmaking p22
Nicole Rusk
doi:10.1038/nmeth1159
Abstract | Full text | PDF
(606K)
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Special Feature: Method of the Year 2007
Structural analysis of macromolecular assemblies p23
Allison Doerr
doi:10.1038/nmeth1160
Abstract | Full text | PDF
(132K)
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Special Feature: Method of the Year 2007
Controlling cells with light p23
Daniel Evanko
doi:10.1038/nmeth1161
Abstract | Full text | PDF
(132K)
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Special Feature: Method of the Year 2007
Top-down mass spectrometry p24
Allison Doerr
doi:10.1038/nmeth1162
Abstract | Full text | PDF
(322K)
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Special Feature: Method of the Year 2007
Induced pluripotency in human cells p24
Natalie de Souza
doi:10.1038/nmeth1163
Abstract | Full text | PDF
(322K)
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Special Feature: Method of the Year 2007
Watching single molecules in cells p25
Daniel Evanko
doi:10.1038/nmeth1164
Abstract | Full text | PDF
(237K)
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Special Feature: Method of the Year 2007
A meta-network of -omics p25
Nicole Rusk
doi:10.1038/nmeth1165
Abstract | Full text | PDF
(237K)
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| News and Views | Top |
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Life from information pp27 - 28
Christopher A Voigt
doi:10.1038/nmeth0108-27
Abstract | Full text | PDF
(128K)
See also: Brief Communication by Itaya et al. | Brief Communication by Bang & Church |
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| Review | Top |
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Live-cell imaging of cAMP dynamics pp29 - 36
Debbie Willoughby & Dermot M F Cooper
Published online: 28 December 2007 | doi:10.1038/nmeth1135
Abstract | Full text | PDF
(386K)
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| Brief Communications | Top |
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Gene synthesis by circular assembly amplification pp37 - 39
Duhee Bang & George M Church
Published online: 25 November 2007 | doi:10.1038/nmeth1136
Abstract | Full text | PDF
(226K)
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See also: News and Views by Voigt |
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Bottom-up genome assembly using the Bacillus subtilis genome vector pp41 - 43
Mitsuhiro Itaya, Kyoko Fujita, Azusa Kuroki & Kenji Tsuge
Published online: 09 December 2007 | doi:10.1038/nmeth1143
Abstract | Full text | PDF
(148K)
| Supplementary Information
See also: News and Views by Voigt |
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In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography pp45 - 47
Claudio Vinegoni, Chrysoula Pitsouli, Daniel Razansky, Norbert Perrimon & Vasilis Ntziachristos
Published online: 09 December 2007 | doi:10.1038/nmeth1149
Abstract | Full text | PDF
(271K)
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Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster pp49 - 51
Jian-Quan Ni, Michele Markstein, Richard Binari, Barret Pfeiffer, Lu-Ping Liu, Christians Villalta, Matthew Booker, Lizabeth Perkins & Norbert Perrimon
Published online: 16 December 2007 | doi:10.1038/nmeth1146
Abstract | Full text | PDF
(225K)
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GraFix: sample preparation for single-particle electron cryomicroscopy pp53 - 55
Berthold Kastner, Niels Fischer, Monika Mariola Golas, Bjoern Sander, Prakash Dube, Daniel Boehringer, Klaus Hartmuth, Jochen Deckert, Florian Hauer, Elmar Wolf, Hannes Uchtenhagen, Henning Urlaub, Franz Herzog, Jan Michael Peters, Dietmar Poerschke, Reinhard Lührmann & Holger Stark
Published online: 23 December 2007 | doi:10.1038/nmeth1139
Abstract | Full text | PDF
(294K)
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Integrating spatially resolved three-dimensional MALDI IMS with in vivo magnetic resonance imaging pp57 - 59
Tuhin K Sinha, Sheerin Khatib-Shahidi, Thomas E Yankeelov, Khubaib Mapara, Moneeb Ehtesham, D Shannon Cornett, Benoit M Dawant, Richard M Caprioli & John C Gore
Published online: 16 December 2007 | doi:10.1038/nmeth1147
Abstract | Full text | PDF
(164K)
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| Articles | Top |
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Targeted patch-clamp recordings and single-cell electroporation of unlabeled neurons in vivo pp61 - 67
Kazuo Kitamura, Benjamin Judkewitz, Masanobu Kano, Winfried Denk & Michael Häusser
Published online: 23 December 2007 | doi:10.1038/nmeth1150
Abstract | Full text | PDF
(3,143K)
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A proteome chip approach reveals new DNA damage recognition activities in Escherichia coli pp69 - 74
Chien-Sheng Chen, Ekaterina Korobkova, Hao Chen, Jian Zhu, Xing Jian, Sheng-Ce Tao, Chuan He & Heng Zhu
Published online: 16 December 2007 | doi:10.1038/nmeth1148
Abstract | Full text | PDF
(411K)
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Resolution of de novo HIV production and trafficking in immature dendritic cells pp75 - 85
Stuart G Turville, Meropi Aravantinou, Hella Stössel, Nikolaus Romani & Melissa Robbiani
Published online: 02 December 2007 | doi:10.1038/nmeth1137
Abstract | Full text | PDF
(1,148K)
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Tracking transmitter-gated P2X cation channel activation in vitro and in vivo pp87 - 93
Esther Richler, Severine Chaumont, Eiji Shigetomi, Alvaro Sagasti & Baljit S Khakh
Published online: 16 December 2007 | doi:10.1038/nmeth1144
Abstract | Full text | PDF
(479K)
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Targeted pre-mRNA modification for gene silencing and regulation pp95 - 100
Xinliang Zhao & Yi-Tao Yu
Published online: 09 December 2007 | doi:10.1038/nmeth1142
Abstract | Full text | PDF
(308K)
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| Protocol | Top |
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Imaging mass spectrometry of proteins and peptides: 3D volume reconstruction pp101 - 108
Malin Andersson, M Reid Groseclose, Ariel Y Deutch & Richard M Caprioli
doi:10.1038/nmeth1145
Abstract | Full text | PDF
(665K)
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| Technology Feature | Top |
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Lab automation: tales along the road to automation pp109 - 112
Nathan Blow
doi:10.1038/nmeth0108-109
Abstract | Full text | PDF
(773K)
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| Errata | Top |
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Erratum: Rapid control of protein level in the apicomplexan Toxoplasma gondii p113
Angelika Herm-Götz, Carolina Agop-Nersesian, Sylvia Münter, Joshua S Grimley, Thomas J Wandless, Friedrich Frischknecht & Markus Meissner
doi:10.1038/nmeth0108-113a
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(72K)
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Erratum: Following the wires p113
Nathan Blow
doi:10.1038/nmeth0108-113b
Full text | PDF
(72K)
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| Application Note | Top |
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MicroRNA profiling on a rapidly evolving microfluidic array
Sonja Vorwerk & Peter Roberts
Abstract | Full text | PDF
(232K)
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