Nature Methods
- 5, 57 - 59 (2008)
Published online: 16 December 2007; | doi:10.1038/nmeth1147
Integrating spatially resolved three-dimensional MALDI IMS with in vivo magnetic resonance imagingTuhin K Sinha1, Sheerin Khatib-Shahidi2, Thomas E Yankeelov1, 3, 4, Khubaib Mapara5, Moneeb Ehtesham5, 6, D Shannon Cornett7, Benoit M Dawant8, Richard M Caprioli2, 7 & John C Gore1, 3, 4, 91
Department of Radiology and Radiological Sciences, Vanderbilt University, 1161 21st Avenue South, Nashville, Tennessee 37232, USA. 2
Department of Chemistry, Vanderbilt University, 1161 21st Avenue South, Nashville, Tennessee 37232, USA. 3
Department of Physics and Astronomy, Vanderbilt University, 1161 21st Avenue South, Nashville, Tennessee 37232, USA. 4
Department of Biomedical Engineering, Vanderbilt University, 1161 21st Avenue South, Nashville, Tennessee 37232, USA. 5
Department of Neurological Surgery, Vanderbilt University, 1161 21st Avenue South, Nashville, Tennessee 37232, USA. 6
Department of Cancer Biology, Vanderbilt University, 1161 21st Avenue South, Nashville, Tennessee 37232, USA. 7
Department of Biochemistry, Vanderbilt University, 1161 21st Avenue South, Nashville, Tennessee 37232, USA. 8
Department of Electrical Engineering and Computer Science, Vanderbilt University, 1161 21st Avenue South, Nashville, Tennessee 37232, USA. 9
Department of Molecular Physiology and Biophysics, Vanderbilt University, 1161 21st Avenue South, Nashville, Tennessee 37232, USA.
Correspondence should be addressed to Tuhin K Sinha tk.sinha@vanderbilt.edu We have developed a method for integrating three dimensional–volume reconstructions of spatially resolved matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) ion images of whole mouse heads with high-resolution images from other modalities in an animal-specific manner. This approach enabled us to analyze proteomic profiles from MALDI IMS data with corresponding in vivo data provided by magnetic resonance imaging.
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