Nature Methods
- 5, 41 - 43 (2008)
Published online: 9 December 2007; | doi:10.1038/nmeth1143
Bottom-up genome assembly using the Bacillus subtilis genome vectorMitsuhiro Itaya1, 2, Kyoko Fujita1, Azusa Kuroki1, 2 & Kenji Tsuge1, 21
Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida-shi, Tokyo 194-8511, Japan. 2
Institute for Advanced Biosciences, Keio University, Nihonkoku, Tsuruoka-shi, Yamagata 997-0017, Japan.
Correspondence should be addressed to Mitsuhiro Itaya mita2001@sfc.keio.ac.jp We established a protocol to construct complete recombinant genomes from their small contiguous DNA pieces and obtained the genomes of mouse mitochondrion and rice chloroplast using a B. subtilis genome (BGM) vector. This method allows the design of any recombinant genomes, valuable not only for fundamental research in systems biology and synthetic biology but also for various applications in the life sciences.
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