Abstract
We established a protocol to construct complete recombinant genomes from their small contiguous DNA pieces and obtained the genomes of mouse mitochondrion and rice chloroplast using a B. subtilis genome (BGM) vector. This method allows the design of any recombinant genomes, valuable not only for fundamental research in systems biology and synthetic biology but also for various applications in the life sciences.
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Acknowledgements
We thank Y. Tozawa, S. Kaneko, I. Yonemura and M. Ikeuchi for helpful discussions. We appreciate the technical help given by Y. Sakuma, and the crude rice DNA samples provided by S. Kosugi and H. Yanagawa (Institute for Advanced Biosciences, Keio Univ.).
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Itaya, M., Fujita, K., Kuroki, A. et al. Bottom-up genome assembly using the Bacillus subtilis genome vector. Nat Methods 5, 41–43 (2008). https://doi.org/10.1038/nmeth1143
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DOI: https://doi.org/10.1038/nmeth1143
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