Nature Methods
- 4, 741 - 746 (2007)
Published online: 26 August 2007; | doi:10.1038/nmeth1083
Bright far-red fluorescent protein for whole-body imagingDmitry Shcherbo1, 3, Ekaterina M Merzlyak2, 3, Tatiana V Chepurnykh2, Arkady F Fradkov1, Galina V Ermakova1, Elena A Solovieva1, Konstantin A Lukyanov1, Ekaterina A Bogdanova1, Andrey G Zaraisky1, Sergey Lukyanov1 & Dmitriy M Chudakov11
Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, 117997 Moscow, Russia. 2
Evrogen JSC, Miklukho-Maklaya 16/10, Moscow 117997, Russia. 3
These authors contributed equally to this work.
Correspondence should be addressed to Dmitriy M Chudakov chudakovdm@mail.ru For deep imaging of animal tissues, the optical window favorable for light penetration is in near-infrared wavelengths, which requires proteins with emission spectra in the far-red wavelengths. Here we report a far-red fluorescent protein, named Katushka, which is seven- to tenfold brighter compared to the spectrally close HcRed or mPlum, and is characterized by fast maturation as well as a high pH-stability and photostability. These unique characteristics make Katushka the protein of choice for visualization in living tissues. We demonstrate superiority of Katushka for whole-body imaging by direct comparison with other red and far-red fluorescent proteins. We also describe a monomeric version of Katushka, named mKate, which is characterized by high brightness and photostability, and should be an excellent fluorescent label for protein tagging in the far-red part of the spectrum.
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