Nature Methods
- 4, 713 - 715 (2007)
Published online: 19 August 2007; | doi:10.1038/nmeth1081
Highly efficient somatic-mutation identification using Escherichia coli mismatch-repair detectionBrock A Peters1, 4, Zhengyan Kan1, 4, Dragan Sebisanovic1, Kanan Pujara1, Zhiyong Wang3, Peter Hong1, Bernard Chow1, Jeremy Stinson1, Victoria E H Carlton3, Thinh Q Pham2, Howard Stern2, Paul Waring2, Kenneth J Hillan2, David A Eberhard2, Frederic de Sauvage1, Jianbiao Zheng3, Malek Faham3 & Somasekar Seshagiri11
Department of Molecular Biology, Genentech, 1 DNA Way, South San Francisco, California 94080, USA. 2
Department of Pathology, Genentech, 1 DNA Way, South San Francisco, California 94080, USA. 3
Affymetrix Inc., 7300 Shoreline Ct., South San Francisco, California 94080, USA. 4
These authors contributed equally to this work.
Correspondence should be addressed to Somasekar Seshagiri sekar@gene.com The discovery of somatic mutations in cancer tissue is extremely laborious, time-consuming and costly. In an evaluation comparing mismatch repair detection (MRD) against Sanger sequencing for somatic-mutation detection, we found that MRD had a specificity of 96% and a sensitivity of 92%. Our results showed that MRD is a robust and cost-effective alternative to Sanger sequencing for identifying somatic mutations in human tumors.
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