Nature Methods
- 4, 717 - 719 (2007)
Published online: 12 August 2007; | doi:10.1038/nmeth1078
Tomographic phase microscopyWonshik Choi1, Christopher Fang-Yen1, Kamran Badizadegan1, 2, Seungeun Oh1, Niyom Lue1, Ramachandra R Dasari1 & Michael S Feld11
G.R. Harrison Spectroscopy Laboratory, 77 Massachusetts Avenue 6-014, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. 2
Department of Pathology, Harvard Medical School and Massachusetts General Hospital, Boston, Massachusetts 02114, USA.
Correspondence should be addressed to Michael S Feld msfeld@mit.edu We report a technique for quantitative three-dimensional (3D) mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. We demonstrate tomographic imaging of cells and multicellular organisms, and time-dependent changes in cell structure. Our results will permit quantitative characterization of specimen-induced aberrations in high-resolution microscopy and have multiple applications in tissue light scattering.
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