Nature Methods
- 4, 555 - 557 (2007)
Published online: 17 June 2007; | doi:10.1038/nmeth1062
Bright monomeric red fluorescent protein with an extended fluorescence lifetimeEkaterina M Merzlyak1, Joachim Goedhart2, Dmitry Shcherbo3, Mariya E Bulina3, Aleksandr S Shcheglov3, Arkady F Fradkov3, Anna Gaintzeva1, Konstantin A Lukyanov3, Sergey Lukyanov3, Theodorus W J Gadella2 & Dmitriy M Chudakov31
Evrogen JSC, Miklukho-Maklaya 16/10, Moscow 117997, Russia. 2
Swammerdam Institute for Life Sciences, Section of Molecular Cytology, Centre for Advanced Microscopy, University of Amsterdam, Kruislaan 316, NL-1098 SM, Amsterdam, The Netherlands. 3
Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, 117997 Moscow, Russia.
Correspondence should be addressed to Dmitriy M Chudakov chudakovdm@mail.ru Fluorescent proteins have become extremely popular tools for in vivo imaging and especially for the study of localization, motility and interaction of proteins in living cells. Here we report TagRFP, a monomeric red fluorescent protein, which is characterized by high brightness, complete chromophore maturation, prolonged fluorescence lifetime and high pH-stability. These properties make TagRFP an excellent tag for protein localization studies and fluorescence resonance energy transfer (FRET) applications.
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