Nature Methods
- 4, 559 - 561 (2007)
Published online: 10 June 2007; | doi:10.1038/nmeth1055
Imaging axonal transport of mitochondria in vivoThomas Misgeld1, 2, 5, Martin Kerschensteiner1, 3, 5, Florence M Bareyre1, 3, Robert W Burgess4 & Jeff W Lichtman11
Department of Molecular and Cellular Biology, Harvard University, 7 Divinity Avenue, Cambridge, Massachusetts 02138, USA. 2
Institute of Neurosciences, Technical University Munich, 80802 Munich, Germany. 3
Institute of Clinical Neuroimmunology, Ludwig-Maximilians University, 81377 Munich, Germany. 4
The Jackson Laboratory, Bar Harbor, Maine 04609, USA. 5
These authors contributed equally to this work.
Correspondence should be addressed to Jeff W Lichtman jeff@mcb.harvard.edu or Thomas Misgeld thomas.misgeld@lrz.tu-muenchen.de Neuronal mitochondria regulate synaptic physiology and cellular survival, and disruption of their function or transport causes neurological disease. We present a fluorescence method to selectively image mitochondrial dynamics in the mouse nervous system, in both live mice and acute explants. We show that axon damage and recovery lead to early and sustained changes in anterograde and retrograde transport. In vivo imaging of mitochondria will be a useful tool to analyze this essential organelle.
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