Nature Methods
- 4, 567 - 569 (2007)
Published online: 3 June 2007; | doi:10.1038/nmeth1053
Nucleic acid and protein mass mapping by live-cell deep-ultraviolet microscopyBenjamin J Zeskind1, 2, 3, Caroline D Jordan1, 4, Winston Timp1, 2, 5, Linda Trapani1, 7, Guichy Waller2, Victor Horodincu1, Daniel J Ehrlich2 & Paul Matsudaira1, 2, 3, 61
WI-MIT BioImaging Center, 500 Technology Square NE47-287, Cambridge, Massachusetts 02142, USA. 2
Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA. 3
Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA. 4
Department of Mathematics, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA. 5
Departments of Electrical Engineering & Computer Science, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA. 6
Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA. 7
Division of Graduate Medical Sciences, Boston University, 715 Albany Street L-317, Boston, Massachusetts 02118, USA.
Correspondence should be addressed to Benjamin J Zeskind bzeskind@alum.mit.edu We developed a deep-ultraviolet (UV) microscope capable of imaging cell mitosis and motility at 280 nm for 45 min with minimal UV-induced toxicity, and for 6 h before the onset of visible cell death in cultured human and mouse cells. Combined with computational methods that convert the intensity of each pixel into an estimate of mass, deep-UV microscopy images generate maps of nucleic acid mass, protein mass and fluorescence yield in unlabeled cells.
MORE ARTICLES LIKE THIS These links to content published by NPG are automatically generated.
|
