Nature Methods
- 4, 487 - 489 (2007)
Published online: 21 May 2007; | doi:10.1038/nmeth1052
Pervasive combinatorial modification of histone H3 in human cellsBenjamin A Garcia1, James J Pesavento2, Craig A Mizzen1, 3 & Neil L Kelleher1, 41
Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois
61801, USA. 2
Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois
61801, USA. 3
Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois
61801, USA. 4
Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois
61801, USA.
Correspondence should be addressed to Craig A Mizzen cmizzen@life.uiuc.edu or Neil L Kelleher kelleher@scs.uiuc.edu We developed a platform using hydrophilic interaction chromatography and high-resolution tandem mass spectrometry (MS) for analyses of histone H3 that allows comprehensive characterization of 'histone codes' at the molecular level. We identified over 150 differentially modified forms of histone H3.2 in asynchronously grown and butyrate-treated HeLa cells, revealing pervasive combinatorial modification previously unaccounted for by other techniques and providing a clarified estimate of the molecular diversity of histone H3 in mammals.
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