Nature Methods
- 4, 501 - 509 (2007)
Published online: 7 May 2007; | doi:10.1038/nmeth1041
Generation of functional hemangioblasts from human embryonic stem cellsShi-Jiang Lu1, Qiang Feng1, Sergio Caballero2, Yu Chen3, Malcolm A S Moore3, Maria B Grant2 & Robert Lanza11
Advanced Cell Technology, Worcester, Massachusetts 01605, USA. 2
Program in Stem Cell Biology and Department of Pharmacology and Therapeutics, University of Florida, Gainesville, Florida 32610, USA. 3
Cell Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA.
Correspondence should be addressed to Robert Lanza rlanza@advancedcell.com Recent evidence suggests the existence of progenitor cells in adult tissues that are capable of differentiating into vascular structures as well as into all hematopoietic cell lineages. Here we describe an efficient and reproducible method for generating large numbers of these bipotential progenitors—known as hemangioblasts—from human embryonic stem (hES) cells using an in vitro differentiation system. Blast cells expressed gene signatures characteristic of hemangioblasts, and could be expanded, cryopreserved and differentiated into multiple hematopoietic lineages as well as into endothelial cells. When we injected these cells into rats with diabetes or into mice with ischemia-reperfusion injury of the retina, they localized to the site of injury in the damaged vasculature and appeared to participate in repair. Injection of the cells also reduced the mortality rate after myocardial infarction and restored blood flow in hind limb ischemia in mouse models. Our data suggest that hES-derived blast cells (hES-BCs) could be important in vascular repair.
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