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Brief Communication
Nature Methods 4, 393–395 (1 May 2007) | doi:10.1038/nmeth1046
Ligand interaction scan: a general method for engineering ligand-sensitive protein alleles
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Abstract
The ligand interaction scan (LIScan) method is a general procedure for engineering small molecule ligand–regulated forms of a protein that is complementary to other 'reverse' genetic and chemical-genetic methods for drug-target validation. It involves insertional mutagenesis by a chemical-genetic 'switch', comprising a genetically encoded peptide module that binds with high affinity to a small-molecule ligand.
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