Nature Methods
- 4, 405 - 407 (2007)
Published online: 22 April 2007; | doi:10.1038/nmeth1042
A yeast two-hybrid smart-pool-array system for protein-interaction mappingFulai Jin1, Larisa Avramova2, Jing Huang1 & Tony Hazbun2, 31
Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, and the Molecular Biology Institute, University of California, Los Angeles, California 90095, USA. 2
The Bindley Bioscience Center, Purdue University, West Lafayette, Indiana 47907, USA. 3
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907, USA.
Correspondence should be addressed to Tony Hazbun thazbun@purdue.edu or Jing Huang jinghuang@mednet.ucla.edu We present here a new two-hybrid smart pool array (SPA) system in which, instead of individual activation domain strains, well-designed activation domain pools are screened in an array format that allows built-in replication and prey-bait deconvolution. Using this method, a Saccharomyces cerevisiae genome SPA increases yeast two-hybrid screening efficiency by an order of magnitude.
MORE ARTICLES LIKE THIS These links to content published by NPG are automatically generated.
|
