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Brief Communication

Nature Methods 4, 323–326 (1 April 2007) | doi:10.1038/nmeth1033

Targeting neural circuitry in zebrafish using GAL4 enhancer trapping

Ethan K Scott , Lindsay Mason , Aristides B Arrenberg , Limor Ziv , Nathan J Gosse , Tong Xiao , Neil C Chi , Kazuhide Asakawa , Koichi Kawakami & Herwig Baier

We present a pilot enhancer trap screen using GAL4 to drive expression of upstream activator sequence (UAS)-linked transgenes in expression patterns dictated by endogenous enhancers in zebrafish. The patterns presented include expression in small subsets of neurons throughout the larval brain, which in some cases persist into adult.