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Brief Communication
Nature Methods 4, 311–313 (1 April 2007) | doi:10.1038/nmeth1017
High-resolution three-dimensional imaging of large specimens with light sheet|[ndash]|based microscopy
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Abstract
We report that single (or selective) plane illumination microscopy (SPIM), combined with a new deconvolution algorithm, provides a three-dimensional spatial resolution exceeding that of confocal fluorescence microscopy in large samples. We demonstrate this by imaging large living multicellular specimens obtained in a three-dimensional cell culture.
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