Nature Methods
- 4, 323 - 326 (2007)
Published online: 18 March 2007; | doi:10.1038/nmeth1033
Targeting neural circuitry in zebrafish using GAL4 enhancer trappingEthan K Scott1, Lindsay Mason1, Aristides B Arrenberg1, Limor Ziv1, Nathan J Gosse1, Tong Xiao1, Neil C Chi2, Kazuhide Asakawa3, Koichi Kawakami3 & Herwig Baier11
Department of Physiology, University of California, San Francisco, California 94158, USA. 2
Department of Biochemistry and Biophysics, University of California, San Francisco, California 94158, USA. 3
Division of Molecular and Developmental Biology, National Institute of Genetics and Department of Genetics, The Graduate University for Advanced Studies (SOKENDAI), Mishima, Shizuoka 411-8540, Japan.
Correspondence should be addressed to Herwig Baier herwig.baier@ucsf.edu We present a pilot enhancer trap screen using GAL4 to drive expression of upstream activator sequence (UAS)-linked transgenes in expression patterns dictated by endogenous enhancers in zebrafish. The patterns presented include expression in small subsets of neurons throughout the larval brain, which in some cases persist into adult. Through targeted photoconversion of UAS-driven Kaede and variegated expression of UAS-driven GFP in single cells, we begin to characterize the cellular components of labeled circuits.
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