Nature Methods
- 4, 327 - 329 (2007)
Published online: 18 March 2007; | doi:10.1038/nmeth1020
Multiplexed protein detection by proximity ligation for cancer biomarker validationSimon Fredriksson1, William Dixon1, Hanlee Ji1, Albert C Koong2, Michael Mindrinos1 & Ronald W Davis11
Stanford Genome Technology Center, Bio-X, 318 Campus Dr., Stanford, California 94305, USA. 2
Department of Radiation Oncology, Stanford, California 94305, USA.
Correspondence should be addressed to Simon Fredriksson simon.fredriksson@stanford.edu or Ronald W Davis dbowe@stanford.edu We present a proximity ligation–based multiplexed protein detection procedure in which several selected proteins can be detected via unique nucleic-acid identifiers and subsequently quantified by real-time PCR. The assay requires a 1- l sample, has low-femtomolar sensitivity as well as five-log linear range and allows for modular multiplexing without cross-reactivity. The procedure can use a single polyclonal antibody batch for each target protein, simplifying affinity-reagent creation for new biomarker candidates.
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