Nature Methods
- 4, 311 - 313 (2007)
Published online: 4 March 2007; | doi:10.1038/nmeth1017
High-resolution three-dimensional imaging of large specimens with light sheet–based microscopyPeter J Verveer1, 3, Jim Swoger1, 3, Francesco Pampaloni1, Klaus Greger1, Marco Marcello2 & Ernst H K Stelzer11
Cell Biology & Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany. 2
Biomedical Structure Group, German Cancer Research Center, Im Neuenheimer Feld 280, 69120, Heidelberg, Germany. 3
Present addresses: Department of Systemic Cell Biology, Max Planck Institute of Molecular Physiology, Otto-Hahn-Strasse 11, 44227, Dortmund, Germany (P.J.V.) and Systems Biology Programme, Centre for Genomic Regulation, C/Dr. Aiguader 88, 08003 Barcelona, Spain (J.S.).
Correspondence should be addressed to Peter J Verveer verveer@mpi-dortmund.mpg.de or Jim Swoger jim.swoger@crg.es or Ernst H K Stelzer stelzer@embl.de We report that single (or selective) plane illumination microscopy (SPIM), combined with a new deconvolution algorithm, provides a three-dimensional spatial resolution exceeding that of confocal fluorescence microscopy in large samples. We demonstrate this by imaging large living multicellular specimens obtained in a three-dimensional cell culture. The ability to rapidly image large samples at high resolution with minimal photodamage provides new opportunities especially for the study of subcellular processes in large living specimens.
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