Reproducible isolation of distinct, overlapping segments of the phosphoproteome

Bernd Bodenmiller, Lukas N Mueller, Markus Mueller, Bruno Domon & Ruedi Aebersold

Supplementary Fig. 1 (pdf 92K)
Amino acid usage of the identified (phospho)peptides compared to the starting material and the used protein database.

Supplementary Fig. 2 (pdf 68K)
The influence of the peptide to resin ratio on the selectivity and percentage of singly phosphorylated peptides in the case of pTiO₂.

Supplementary Fig. 3 (pdf 24K)
The influence of the peptide to resin ratio on the selectivity and percentage of singly phosphorylated peptides in the case of IMAC.

Supplementary Table 1 (pdf 100K)
Values of the similarity and overlap between any two LC-MS runs as computed by Superhirn.

Supplementary Table 2 (pdf 216K)
Phosphopeptides identified with PAC, IMAC, pTiO₂ and dhbTiO₂.

Supplementary Table 3 (pdf 160K)
Influence of methylation prior and/or after phosphopeptide isolation using IMAC.

Supplementary Methods (pdf 96K)

Top