Nature Methods
- 4, 135 - 137 (2006)
Published online: 17 December 2006; | doi:10.1038/nmeth974
Sensitive protein detection via triple-binder proximity ligation assaysEdith Schallmeiner1, Elli Oksanen1, Olle Ericsson1, Lena Spångberg1, Susann Eriksson2, Ulf-Håkan Stenman3, Kim Pettersson2 & Ulf Landegren11
Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Se-75185 Uppsala, Sweden. 2
Department of Biotechnology, University of Turku, FI-20520, Turku, Finland. 3
Department of Clinical Chemistry, Helsinki University Central Hospital, FI-00290, Helsinki, Finland.
Correspondence should be addressed to Edith Schallmeiner edith.schallmeiner@genpat.uu.se The detection of weakly expressed proteins and protein complexes in biological samples represents a fundamental challenge. We have developed a new proximity-ligation strategy named 3PLA that uses three recognition events for the highly specific and sensitive detection of as little as a hundred molecules of the vascular endothelial growth factor (VEGF), the biomarkers troponin I, and prostate-specific antigen (PSA) alone or in complex with an inhibitor—demonstrating the versatility of 3PLA.
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