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Brief Communication

Nature Methods 4, 927–929 (1 November 2007) | doi:10.1038/nmeth1112

A monoclonal antibody for G protein|[ndash]|coupled receptor crystallography

Peter W Day , S|[oslash]|ren G F Rasmussen , Charles Parnot , Juan Jos|[eacute]| Fung , Asna Masood , Tong Sun Kobilka , Xiao-Jie Yao , Hee-Jung Choi , William I Weis , Daniel K Rohrer & Brian K Kobilka

G protein–coupled receptors (GPCRs) constitute the largest family of signaling proteins in mammals, mediating responses to hormones, neurotransmitters, and senses of sight, smell and taste. Mechanistic insight into GPCR signal transduction is limited by a paucity of high-resolution structural information.