Nature Methods
- 4, 903 - 905 (2007)
Published online: 14 October 2007; Corrected online: 21 October 2007 | doi:10.1038/nmeth1111
Direct selection of human genomic loci by microarray hybridizationThomas J Albert1, Michael N Molla1, Donna M Muzny2, Lynne Nazareth2, David Wheeler2, Xingzhi Song2, Todd A Richmond1, Chris M Middle1, Matthew J Rodesch1, Charles J Packard1, George M Weinstock2 & Richard A Gibbs21
NimbleGen Systems Inc., 1 Science Court, Madison, Wisconsin 53711, USA. 2
Human Genome Sequencing Center, One Baylor Plaza, Houston, Texas 77030, USA.
Correspondence should be addressed to Thomas J Albert talbert@nimblegen.com or Richard A Gibbs agibbs@bcm.tmc.edu We applied high-density microarrays to the enrichment of specific sequences from the human genome for high-throughput sequencing. After capture of 6,726 approximately 500-base 'exon' segments, and of 'locus-specific' regions ranging in size from 200 kb to 5 Mb, followed by sequencing on a 454 Life Sciences FLX sequencer, most sequence reads represented selection targets. These direct selection methods supersede multiplex PCR for the large-scale analysis of genomic regions.
NOTE: In the version of this article initially published online, e-mail address of the corresponding author was incorrect. The correct address should be talbert@nimblegen.com. The error has been corrected for all versions of the article.
MORE ARTICLES LIKE THIS These links to content published by NPG are automatically generated.
|
