Nature Methods
- 4, 943 - 950 (2007)
Published online: 28 October 2007; | doi:10.1038/nmeth1105
Two-photon photostimulation and imaging of neural circuitsVolodymyr Nikolenko, Kira E Poskanzer & Rafael Yuste
Howard Hughes Medical Institute, Department of Biological Sciences, Columbia University, 1212 Amsterdam Avenue, Box 2435, New York, New York 10027, USA.
Correspondence should be addressed to Volodymyr Nikolenko vn59@columbia.edu We introduce an optical method to stimulate individual neurons in brain slices in any arbitrary spatiotemporal pattern, using two-photon uncaging of MNI-glutamate with beam multiplexing. This method has single-cell and three-dimensional precision. By sequentially stimulating up to a thousand potential presynaptic neurons, we generated detailed functional maps of inputs to a cell. We combined this approach with two-photon calcium imaging in an all-optical method to image and manipulate circuit activity.
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