Nature Methods
- 4, 919 - 921 (2007)
Published online: 30 September 2007; | doi:10.1038/nmeth1090
An activity-independent selection system of thermostable protein variantsHélène Chautard1, Emilio Blas-Galindo2, Thierry Menguy1, Laure Grand'Moursel1, Felipe Cava2, José Berenguer2 & Marc Delcourt11
Biométhodes SA, Genavenir 8, 5 rue Henri Desbruères, 91030 Evry, France. 2
Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid 28049 Madrid, Spain.
Correspondence should be addressed to José Berenguer jberenguer@cbm.uam.es or Marc Delcourt marc.delcourt@biomethodes.com We describe an activity-independent method for the selection of thermostable mutants of any protein. It is based on a fusion construct comprising the protein of interest and a thermostable antibiotic resistance reporter, in such a way that thermostable mutants provide increased resistance in a thermophile. We isolated thermostable mutants of three human interferons and of two enzymes to demonstrate the applicability of the system.
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