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Brief Communication

Nature Methods 4, 835–837 (1 October 2007) | doi:10.1038/nmeth1091

MegaPlex PCR: a strategy for multiplex amplification

Linda Str|[ouml]|mqvist Meuzelaar , Owen Lancaster , J Paul Pasche , Guido Kopal & Anthony J Brookes

'MegaPlex PCR' is a robust technology for highly multiplexed amplification of specific DNA sequences. It uses target-specific pairs of PCR primers that are physically separated by surface immobilization.