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Brief Communication

Nature Methods 4, 839–841 (1 October 2007) | doi:10.1038/nmeth1084

Speed-mapping quantitative trait loci using microarrays

Chao-Qiang Lai , Jeff Leips , Wei Zou , Jessica F Roberts , Kurt R Wollenberg , Laurence D Parnell , Zhao-Bang Zeng , Jose M Ordovas & Trudy F C Mackay

We developed a rapid, economical method for high-resolution quantitative trait locus (QTL) mapping using microarrays for selective genotyping of pooled DNA samples. We generated 21,207 F2 flies from two inbred Drosophila melanogaster strains with known QTLs affecting lifespan, and hybridized DNA pools of young and old flies to microarrays.