Nature Methods
- 4, 835 - 837 (2007)
Published online: 16 September 2007; | doi:10.1038/nmeth1091
MegaPlex PCR: a strategy for multiplex amplificationLinda Strömqvist Meuzelaar1, Owen Lancaster1, J Paul Pasche2, Guido Kopal2 & Anthony J Brookes11
Department of Genetics, University of Leicester, University Road, Leicester, LE1 7RH, UK. 2
Roche Applied Science, Nonnenwald 2, 82372 Penzberg, Germany.
Correspondence should be addressed to Anthony J Brookes ajb97@leicester.ac.uk 'MegaPlex PCR' is a robust technology for highly multiplexed amplification of specific DNA sequences. It uses target-specific pairs of PCR primers that are physically separated by surface immobilization. Initial surface-based amplification cycles are then coupled to efficient solution-phase PCR using one common primer pair. We demonstrate this method by co-amplifying and genotyping 75 unselected human single-nucleotide polymorphism (SNP) loci.
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