Fast manipulation of cellular cAMP level by light in vivo

Saskia Schröder-Lang, Martin Schwärzel, Reinhard Seifert, Timo Strünker, Suneel Kateriya, Jens Looser, Masakatsu Watanabe, U Benjamin Kaupp, Peter Hegemann & Georg Nagel

Supplementary Fig. 1 (pdf 52K)
Detection of PAC and PAC in oocytes.

Supplementary Fig. 2 (pdf 40K)
Light-dependence of PAC activation.

Supplementary Fig. 3 (pdf 120K)
Photostimulation of PAC does not alter behavior in freely moving Drosophila.

Supplementary Table 1 (pdf 72K)
ON- and OFF kinetics of light-induced behavioral changes for two consecutive irradiations of 1 min.

Supplementary Video 1 (mov 872K)
Change in grooming behavior of PAC expressing flies (elav-Gal4 / UAS-PAC) upon illumination with bright blue light. An individual fly is illuminated during grooming behavior (note the diode placed underneath the observation chamber turning on) resulting in nearly instant stop of activity and unusual leg shaking. After turning off blue light the animal returns to grooming activity. Note that the video was taped using a red filter that extinguishes blue light to protect the camera; blue light intensity was set to 55.7 microMol (m^-2 s^-1).

Supplementary Video 2 (mov 268K)
Illumination with blue light does not alter grooming behavior in wild type Canton-S flies.

Supplementary Video 3 (mov 1M)
Illumination with amber light (590 10 nm) does not alter grooming activity in Pac expressing flies (elav-Gal4 / UAS-PAC). Note that the video was taped using a blue filter that partly extinguishes amber light to protect the camera; light intensity was set to 55.7 microMol (m^-2 s^-1).

Supplementary Data (pdf 80K)

Supplementary Methods (pdf 104K)

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