 | Figure 1
Nature Methods
- 4, 39 - 42 (2007)
Published online: 26 November 2006; | doi:10.1038/nmeth975
Fast manipulation of cellular cAMP level by light in vivoSaskia Schröder-Lang, Martin Schwärzel, Reinhard Seifert, Timo Strünker, Suneel Kateriya, Jens Looser, Masakatsu Watanabe, U Benjamin Kaupp, Peter Hegemann & Georg Nagel | | | | Figure 1. Expression of PACs in oocytes.
(a) Total [cAMP], 4 d after injection of cRNA. Oocytes were kept in the dark or irradiated for 5 min with blue light (380–480 nm). Control, noninjected oocytes. Error bars, mean  s.d. (b) Oocytes expressing CFTR and PAC (200 pg cRNA, left) or PAC (20 ng cRNA, right). Bars indicate duration of drug application (red) or light pulse (blue, black). Conductance (G) is plotted against time. We applied 0.5 mM IBMX, 10  M forskolin to a PAC and CFTR–expressing oocyte (red trace), and the same oocyte was irradiated with blue light (blue trace; left). (c) CFTR current upon high-intensity irradiation (from a light-emitting diode with 28 mmole photons m-2 s-1) of a PAC-expressing oocyte. (d) Summary of light-induced conductance changes when expressing PACs with CFTR. Control = not injected. The amount of PAC-cRNA injected and the number of experiments is indicated below and above the bars, respectively. Student's t-test: at the 0.01 level, the difference of the population means is significant for control and PAC, for control and 20 ng PAC, and for control and PAC-PAC; the difference of the population means is not significant for control and CFTR, for control and 200 pg PAC , for 200 pg PAC and 20 ng PAC, and for PAC and PAC-PAC. (e) Coexpression of PAC with CNGA2-T537S (left), of PAC with CNGA2-C460W,E583M (right). Light pulses indicated by bars. (f) Photoactivated inward current at –60 mV of an oocyte expressing PAC with CNGA2-C460W,E583M.
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