Nature Methods
- 4, 31 - 33 (2007)
Published online: 3 December 2006; | doi:10.1038/nmeth985
Tomographic molecular imaging and 3D quantification within adult mouse organsTomas Alanentalo1, 4, Amir Asayesh1, 4, Harris Morrison2, Christina E Lorén1, Dan Holmberg3, James Sharpe2, 5 & Ulf Ahlgren11
Umeå Center for Molecular Medicine, Umeå University, S-901 87, Umeå, Sweden. 2
MRC Human Genetics Unit, Western General Hospital, Crewe Rd., Edinburgh EH4 2XU, UK. 3
Department of Medical and Clinical Genetics, Umeå University, S-901 85, Umeå, Sweden. 4
These authors contributed equally to this work. 5
Present address: Center for Genomic Regulation, Passeig Marítim, 37-49, 08003, Barcelona, Spain.
Correspondence should be addressed to James Sharpe james.sharpe@crg.es or Ulf Ahlgren ulf.ahlgren@ucmm.umu.se A convenient technology to quantify three-dimensional (3D) morphological features would have widespread applications in biomedical research. Based on combined improvements in sample preparation, tomographic imaging and computational processing, we present a procedure for high-resolution 3D quantification of structures within intact adult mouse organs. Using the nonobese diabetic (NOD) mouse model, we demonstrate a correlation between total islet  -cell volume and the onset of type-1 diabetes.
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