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Brief Communication
Nature Methods - 4, 39 - 42 (2007)
Published online: 26 November 2006; | doi:10.1038/nmeth975

Fast manipulation of cellular cAMP level by light in vivo

Saskia Schröder-Lang1, Martin Schwärzel2, Reinhard Seifert3, Timo Strünker3, Suneel Kateriya4, Jens Looser5, Masakatsu Watanabe6, 7, U Benjamin Kaupp3, Peter Hegemann4 & Georg Nagel1, 5

1  Max-Planck-Institut für Biophysik, 60439 Frankfurt, Germany.

2  Saarland Universität, Naturwissenschaftlich-Technische Fakultät III, FR 8.3, Zoologie & Physiologie, 66041 Saarbrücken, Germany.

3  Forschungszentrum Jülich, Institut für Neurowissenschaften und Biophysik, Abteilung Zelluläre Signalverarbeitung, INB-1, 52425 Jülich, Germany.

4  Humboldt Universität zu Berlin, Experimentelle Biophysik, 10115 Berlin, Germany.

5  Universität Würzburg, Julius-von-Sachs-Institut für Biowissenschaften, 97082 Würzburg, Germany.

6  National Institute for Basic Biology, Okazaki, 444-8585, Japan.

7  School of Advanced Sciences, Graduate University of Advanced Studies (SOKENDAI), Hayama, 240-0193, Japan.

Correspondence should be addressed to Georg Nagel nagel@botanik.uni-wuerzburg.de or Martin Schwärzel m.schwaerzel@mx.uni-saarland.de

The flagellate Euglena gracilis contains a photoactivated adenylyl cyclase (PAC), consisting of the flavoproteins PACalpha and PACbeta. Here we report functional expression of PACs in Xenopus laevis oocytes, HEK293 cells and in Drosophila melanogaster, where neuronal expression yields light-induced changes in behavior. The activity of PACs is strongly and reversibly enhanced by blue light, providing a powerful tool for light-induced manipulation of cAMP in animal cells.

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Nature Methods
ISSN: 1548-7091
EISSN: 1548-7105
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