 |
| In This Issue |  Top |
|
 |
|
| PDF |
|
| Focus | Top |
|
|
|
|
|
| A set of four Perspectives addresses the most common practical questions surrounding the use of RNAi in mammalian cells, and is followed by four Perspectives on currently available RNAi libraries for genome-wide RNAi screens. This focus is available free until the end of November. |
|
| Editorial | Top |
|
|
|
RNA interference on target p659
doi:10.1038/nmeth0906-659
Full text | PDF
(49K)
|
|
| Research Highlights | Top |
|
|
|
STEDy progress p661
Daniel Evanko
doi:10.1038/nmeth0906-661
Abstract | Full text | PDF
(96K)
|
|
|
|
Quantum dots go with the flow pp662 - 663
Irene Kaganman
doi:10.1038/nmeth0906-662a
Abstract | Full text | PDF
(131K)
|
|
|
|
|
|
|
|
In praise of manual high-throughput screening pp662 - 663
Daniel Evanko
doi:10.1038/nmeth0906-662b
Abstract | Full text | PDF
(131K)
|
|
|
|
News In Brief p663
doi:10.1038/nmeth0906-663
Full text | PDF
(66K)
|
|
|
|
Pushing size to the limit: extreme DNA p664
Allison Doerr
doi:10.1038/nmeth0906-664
Abstract | Full text | PDF
(194K)
|
|
| News and Views | Top |
|
|
|
Amplify this! DNA and RNA get a third base pair pp667 - 668
Aaron M Leconte & Floyd E Romesberg
doi:10.1038/nmeth0906-667
Abstract | Full text | PDF
(198K)
See also: Article by Hirao et al. |
|
| Foreword | Top |
|
|
|
Focus on RNA interference - A user's guide
Focus on RNA interference p669
Veronique Kiermer
doi:10.1038/nmeth0906-669
Full text | PDF
(112K)
|
|
| Perspectives | Top |
|
|
|
Focus on RNA interference - A user's guide
On the art of identifying effective and specific siRNAs pp670 - 676
Yi Pei & Thomas Tuschl
Published online: 23 August 2006 | doi:10.1038/nmeth911
Abstract | Full text | PDF
(215K)
|
|
|
|
Focus on RNA interference - A user's guide
Enhancing and confirming the specificity of RNAi experiments pp677 - 681
Bryan R Cullen
Published online: 23 August 2006 | doi:10.1038/nmeth913
Abstract | Full text | PDF
(202K)
|
|
|
|
Focus on RNA interference - A user's guide
Tuning silence: conditional systems for RNA interference pp682 - 688
Maciej Wiznerowicz, Jolanta Szulc & Didier Trono
Published online: 23 August 2006 | doi:10.1038/nmeth914
Abstract | Full text | PDF
(345K)
|
|
|
|
Focus on RNA interference - A user's guide
Expressing short hairpin RNAs in vivo pp689 - 695
Ola Snøve Jr & John J Rossi
Published online: 23 August 2006 | doi:10.1038/nmeth927
Abstract | Full text | PDF
(160K)
|
|
|
|
Focus on RNA interference - A user's guide
Enzymatically prepared RNAi libraries pp696 - 700
Frank Buchholz, Ralf Kittler, Mikolaj Slabicki & Mirko Theis
doi:10.1038/nmeth912
Abstract | Full text | PDF
(165K)
|
|
|
|
Focus on RNA interference - A user's guide
shRNA libraries and their use in cancer genetics pp701 - 706
René Bernards, Thijn R Brummelkamp & Roderick L Beijersbergen
Published online: 23 August 2006 | doi:10.1038/nmeth921
Abstract | Full text | PDF
(320K)
|
|
|
|
Focus on RNA interference - A user's guide
Lessons from Nature: microRNA-based shRNA libraries pp707 - 714
Kenneth Chang, Stephen J Elledge & Gregory J Hannon
Published online: 23 August 2006 | doi:10.1038/nmeth923
Abstract | Full text | PDF
(222K)
| Supplementary Information |
|
|
|
Focus on RNA interference - A user's guide
Genome-scale loss-of-function screening with a lentiviral RNAi library pp715 - 719
David E Root, Nir Hacohen, William C Hahn, Eric S Lander & David M Sabatini
Published online: 23 August 2006 | doi:10.1038/nmeth924
Abstract | Full text | PDF
(197K)
|
|
| Brief Communications | Top |
|
|
|
Nanoscale resolution in GFP-based microscopy pp721 - 723
Katrin I Willig, Robert R Kellner, Rebecca Medda, Birka Hein, Stefan Jakobs & Stefan W Hell
Published online: 23 August 2006 | doi:10.1038/nmeth922
Abstract | Full text | PDF
(392K)
| Supplementary Information |
|
|
|
Digital quantification using amplified single-molecule detection pp725 - 727
Jonas Jarvius, Jonas Melin, Jenny Göransson, Johan Stenberg, Simon Fredriksson, Carlos Gonzalez-Rey, Stefan Bertilsson & Mats Nilsson
Published online: 23 August 2006 | doi:10.1038/nmeth916
Abstract | Full text | PDF
(178K)
| Supplementary Information |
|
| Articles | Top |
|
|
|
An unnatural hydrophobic base pair system: site-specific incorporation of nucleotide analogs into DNA and RNA pp729 - 735
Ichiro Hirao, Michiko Kimoto, Tsuneo Mitsui, Tsuyoshi Fujiwara, Rie Kawai, Akira Sato, Yoko Harada & Shigeyuki Yokoyama
Published online: 23 August 2006 | doi:10.1038/nmeth915
Abstract | Full text | PDF
(404K)
| Supplementary Information
See also: News and Views by Leconte & Romesberg |
|
|
|
Quantitative multiplexed profiling of cellular signaling networks using phosphotyrosine-specific DNA-tagged SH2 domains pp737 - 744
Kevin Dierck, Kazuya Machida, Anja Voigt, Julian Thimm, Martin Horstmann, Walter Fiedler, Bruce J Mayer & Peter Nollau
Published online: 23 August 2006 | doi:10.1038/nmeth917
Abstract | Full text | PDF
(479K)
| Supplementary Information |
|
|
|
A new two-color Fab labeling method for autoantigen protein microarrays pp745 - 751
Michael G Kattah, Golnaz R Alemi, Donna L Thibault, Imelda Balboni & Paul J Utz
Published online: 23 August 2006 | doi:10.1038/nmeth910
Abstract | Full text | PDF
(348K)
| Supplementary Information |
|
| Technology Feature | Top |
|
|
|
'Chain gang' delivers for hard labor pp753 - 762
Michael Eisenstein
doi:10.1038/nmeth0906-753
Abstract | Full text | PDF
(1,208K)
|
|
| Erratum | Top |
|
|
|
Erratum: Efficient site-specific integration in Plasmodium falciparum chromosomes mediated by mycobacteriophage Bxb1 integrase p763
Louis J Nkrumah, Rebecca A Muhle, Pedro A Moura, Pallavi Ghosh, Graham F Hatfull, William R Jacobs Jr & David A Fidock
doi:10.1038/nmeth0906-763
Full text | PDF
(50K)
|
|
| Application Notes | Top |
|
|
|
Multiple-laser microplate cytometers: adding a third dimension to high-content screening
Wayne P Bowen & Sarah L Payne
Published online: 23 August 2006 | doi:10.1038/nmeth918
Abstract | Full text | PDF
(196K)
|
|
|
|
Optimizing knockdown of gene expression using the TriFECTaTM Dicer-substrate RNAi reagent system
Scott D Rose, Michael A Collingwood & Mark A Behlke
Published online: 23 August 2006 | doi:10.1038/nmeth919
Abstract | Full text | PDF
(239K)
|
|
|
|
miRCURYTM LNA research tools for microRNA
Peter Roberts, Mikkel Noerholm, Nina Ståhlberg, Peter Mouritzen & Christian Glue
Published online: 23 August 2006 | doi:10.1038/nmeth920
Abstract | Full text | PDF
(208K)
|
|
|
Top |
|
|
