Nature Methods
- 3, 721 - 723 (2006)
Published online: 23 August 2006; | doi:10.1038/nmeth922
Nanoscale resolution in GFP-based microscopyKatrin I Willig, Robert R Kellner, Rebecca Medda, Birka Hein, Stefan Jakobs & Stefan W Hell
Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Göttingen, Germany.
Correspondence should be addressed to Stefan W Hell shell@gwdg.de We report attainment of subdiffraction resolution using stimulated emission depletion (STED) microscopy with GFP-labeled samples. The  70 nm lateral resolution attained in this study is demonstrated by imaging GFP-labeled viruses and the endoplasmic reticulum (ER) of a mammalian cell. Our results mark the advent of nanoscale biological microscopy with genetically encoded markers.
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