Nature Methods
- 3, 696 - 700 (2006)
doi:10.1038/nmeth912
Enzymatically prepared RNAi librariesFrank Buchholz, Ralf Kittler, Mikolaj Slabicki & Mirko Theis
Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D-01307 Dresden, Germany.
Correspondence should be addressed to Frank Buchholz buchholz@mpi-cbg.de Large-scale RNA interference (RNAi) screens in mammalian cells have mainly used synthetic small interfering RNA (siRNA) or short hairpin RNA (shRNA) libraries. The RNAi triggers for both of these approaches were designed with algorithm-based predictions to identify single sequences for mRNA knockdown. Alternatives to these approaches have recently been developed using enzymatic methods. Here we describe the concepts of enzymatically prepared shRNA and siRNA libraries, and discuss their strengths and limitations.
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