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Figure 3

Nature Methods 3, 609 - 614 (2006)
Published online: 21 July 2006; | doi:10.1038/nmeth899

High-throughput screening methodology for the directed evolution of glycosyltransferases

Amir Aharoni, Karena Thieme, Cecilia P C Chiu, Sabrina Buchini, Luke L Lairson, Hongming Chen, Natalie C J Strynadka, Warren W Wakarchuk & Stephen G Withers

 
Fig 3 full size
Figure 3. Cell-based ST assay for cells expressing wild-type CstII (WT) and containing the pUC18 plasmid (control).
The different cell samples were incubated with Neu5Ac and bodipy-lactose or bodipy-galactose. After extensive washing the cells were analyzed either visually or by FACS. (a) Eppendorfs containing the four different cell samples as visualized under an ultraviolet light lamp; the cell fluorescence intensity is correlated to the CstII activity. (b) FACS histogram analysis of the four different cell samples. Overlapping light blue and light green, cells expressing empty pUC18 plasmid incubated with bodipy-galactose and bodipy-lactose respectively; brown, cells expressing wild-type CstII incubated with bodipy-galactose; purple, cells expressing wild-type CstII incubated with bodipy-lactose.

 
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